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Titlebook: Bacterial Chemosensing; Methods and Protocol Michael D. Manson Book 2018 Springer Science+Business Media, LLC 2018 Chemoreceptor.Chemotaxis

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发表于 2025-3-21 16:20:37 | 显示全部楼层 |阅读模式
期刊全称Bacterial Chemosensing
期刊简称Methods and Protocol
影响因子2023Michael D. Manson
视频video
发行地址Includes cutting-edge methods and protocols for the study of bacterial chemosensing.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from th
学科分类Methods in Molecular Biology
图书封面Titlebook: Bacterial Chemosensing; Methods and Protocol Michael D. Manson Book 2018 Springer Science+Business Media, LLC 2018 Chemoreceptor.Chemotaxis
影响因子This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as .Escherichia coli. and .Salmonella typhimurium., while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful .Methods in Molecular Biology. series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. .Authoritative and cutting-edge, .Bacterial Chemosensing: Methods and Protocols. provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, a
Pindex Book 2018
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发表于 2025-3-21 23:16:56 | 显示全部楼层
Demography and Death by Violenceacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in .. This assay has been used to investigate the mechanism of signal transduction in . chemotaxis and the disparate mechanisms employed by this bacterium for sensory adaptation and gradient sensing.
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In Vitro Assay for Measuring Receptor-Kinase Activity in the , Chemotaxis Pathwayacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in .. This assay has been used to investigate the mechanism of signal transduction in . chemotaxis and the disparate mechanisms employed by this bacterium for sensory adaptation and gradient sensing.
发表于 2025-3-22 10:20:07 | 显示全部楼层
Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interfaceg within the aspartate chemoreceptor of . (Tar). We have also employed the method within other related proteins, such as sensor histidine kinases (SHKs), and therefore hope that other research groups find it useful to modulate signal output from their receptor of interest.
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Carolina Hausmann-Stabile,Lauren E. Gulbasication of this assay that uses a hydrogel matrix to enable quantitative time-course measurements by analyzing image pixel intensities. This approach allows a high-throughput method when coupled with the aid of a motorized microscope stage.
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Kseniya Katsman,Elizabeth L. Jeglicasuring the response of cells to the gradient without exposing them to any flow. Unlike other methods described in the literature, this method is capable of producing gradients of any shape, almost instantaneously, allowing the measurement of time-dependent response of cells to a variety of signals.
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Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillariesication of this assay that uses a hydrogel matrix to enable quantitative time-course measurements by analyzing image pixel intensities. This approach allows a high-throughput method when coupled with the aid of a motorized microscope stage.
发表于 2025-3-23 07:43:42 | 显示全部楼层
A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradienasuring the response of cells to the gradient without exposing them to any flow. Unlike other methods described in the literature, this method is capable of producing gradients of any shape, almost instantaneously, allowing the measurement of time-dependent response of cells to a variety of signals.
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