Titlebook: Bacterial Artificial Chromosomes; Kumaran Narayanan Book 2015Latest edition Springer Science+Business Media New York 2015 BAC construction

烤架

Recombining Overlapping BACs into Single Large BACsd by alternating use of the pBACLInk vectors, resulting in several BAC clones containing as much of the genomic region of a gene as required. Such BACs can then be used in gene expression studies and/or gene therapy applications.

MAZE

A Recombineering-Based Gene Tagging System for Arabidopsisox in many model organisms. In this chapter, we describe the experimental procedures involved in recombineering-based tagging of an Arabidopsis gene contained in a plant transformation-ready bacterial artificial chromosome (TAC).

Ophthalmoscope

BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studiesuccessfully applied in our laboratories many times. BAC recombineering in zebrafish allows for economical functional genomics studies, for example by integrating developmental biology with comparative genomics approaches to validate potential enhancer elements of vertebrate transcription factors.

保存

Charade

genuine

Shape Analysis of Functional Dataorts that target a genomic segment. This chapter describes the sub-genome assembly protocol for an organism based upon a BAC tiling path derived from a genome-scale physical map or from fine mapping using BACs to target sub-genomic regions. Methods that are described include BAC isolation and mapping, DNA sequencing, and sequence assembly.

FLIC

积云

Use Case of Palm Vein Authenticationthrough .-mediated transformation into . species where an intact copy stably integrated into the genome. By modifying the recombineering vector, this method can theoretically be adapted for many different fungi.

jungle

Binocular Vision and Depth Perception. In the latter, rare FS are expressed much more frequently than in, for example, aphidicolin-induced blood lymphocyte preparations. Knowing the exact location of FS enables the molecular comparison of their location and breakpoints that appear during evolution, cancer development and inherited disorders.

Eclampsia

BAC Sequencing Using Pooled Methodsorts that target a genomic segment. This chapter describes the sub-genome assembly protocol for an organism based upon a BAC tiling path derived from a genome-scale physical map or from fine mapping using BACs to target sub-genomic regions. Methods that are described include BAC isolation and mapping, DNA sequencing, and sequence assembly.