Titlebook: Bacterial Artificial Chromosomes; Kumaran Narayanan Book 2015Latest edition Springer Science+Business Media New York 2015 BAC construction

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Infectious Delivery of Alphaherpesvirus Bacterial Artificial Chromosomesnome. (2) Introduction of the transfer plasmid sequences into the alphaherpesvirus genome via homologous recombination in mammalian cells. (3) Isolation of recombinant virus genomes containing the BAC backbone sequences from infected mammalian cells and electroporation into .. (4) Preparation of inf

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New BAC Probe Set to Narrow Down Chromosomal Breakpoints in Small and Large Derivative Chromosomes, be applied on large inborn or acquired derivative chromosomes. The main feature of this set is that the probes are applied in a chromosome-specific manner and they align along the chromosome in average intervals of ten megabasepairs. Hence PCL-FISH provides denser coverage and a more precise anchor

Airtight

1064-3745 o troubleshoot and avoid known pitfalls..Authoritative and cutting-edge, .Bacterial Artificial Chromosomes, Second Edition .seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies..978-1-4939-5430-8978-1-4939-1652-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

REP

Philipp Grohs,Martin Holler,Andreas Weinmannial Chromosomes (YACs) in yeast. The method has a broad application for structural and functional genomics, long-range haplotyping, characterization of chromosomal rearrangements, and evolutionary studies. In this paper, we describe a basic protocol for gene isolation by TAR as well as a method to c

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https://doi.org/10.1007/978-3-030-31351-7its sequence or size. One novel application of recombineering is the assembly of linear BACs in . that can replicate autonomously as linear plasmids. A circular BAC is inserted with a short telomeric sequence from phage N15, which is subsequently cut and rejoined by the phage protelomerase enzyme to

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Handbook of Vascular Biology Techniquesrrying a loxP or a lox511 site is inserted at random into BAC DNA inside the bacterial cell. The cells are then infected with bacteriophage P1. The Cre protein expressed by phage P1 generates end-deletions by specifically recombining the inserted loxP (or lox511) with the loxP (or lox511) endogenous