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Titlebook: Bacterial Artificial Chromosomes; Kumaran Narayanan Book 2015Latest edition Springer Science+Business Media New York 2015 BAC construction

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Kumaran NarayananIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180255.jpg
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978-1-4939-5430-8Springer Science+Business Media New York 2015
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Philipp Grohs,Martin Holler,Andreas Weinmann to allow subsequent gene loading into a unique loxP site of the HAC-based (Human Artificial Chromosome) gene delivery vector. The benefit of combining the TAR gene cloning technology with the HAC gene delivery system for gene expression studies is discussed.
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Vedrana Krivokuća,Sébastien Marceld by alternating use of the pBACLInk vectors, resulting in several BAC clones containing as much of the genomic region of a gene as required. Such BACs can then be used in gene expression studies and/or gene therapy applications.
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Is Smith a Real Virtue Ethicist?uccessfully applied in our laboratories many times. BAC recombineering in zebrafish allows for economical functional genomics studies, for example by integrating developmental biology with comparative genomics approaches to validate potential enhancer elements of vertebrate transcription factors.
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