Titlebook: B Cell Protocols; Hua Gu,Klaus Rajewsky Book 2004 Humana Press 2004

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Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo,from single cells within the population to estimate the level of somatic hypermutation as the best molecular indicator of B-cell memory (.). In this chapter, we describe the methods used in each of these four sections that serve to provide high-resolution quantification of antigen-specific B-cell me

outset

B-Cell Signal Transduction,size the use of phospho-specific antibodies (Abs) and low-molecular-weight enzyme inhibitors in the process of mapping BCR-activated signaling pathways as well as determining activation states of signaling proteins.

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