Titlebook: B Cell Protocols; Hua Gu,Klaus Rajewsky Book 2004 Humana Press 2004

Guileless

节省

Neonatal

围裙

Synthetic Fingerprint Generation, proteins that can serve as markers for recognizing distinct stages in this process. These cell fractions range from just prior to initial heavy chain rearrangement, the germline pro-B stage, through D-J rearranged pro-B and heavy chain expressing pre-B stages, to the maturing surface BCR positive B

GLOSS

Fingerprint Analysis and Representation,are recent bone marrow emigrants traveling with the blood to the spleen. Here they further develop to transitional 2 and mature B cells. Marginal zone B cells are a sessile population only found in the spleen. The distinction of these cell types is only possible by three- and four-color flow cytomet

Cocker

Synthetic Fingerprint Generation,e identification of additional subset markers and the extension of fluorescence-activated cell sorter (FACS) capabilities to simultaneously measure the expression of more markers on individual cells. For example, introduction of a 6–8 multiparameter FACS instrument resulted in significant advances i

Amendment

Davide Maltoni,Dario Maio,Salil Prabhakarques employed, labeling of dividing cells with the thymidine analog 5-bromo-2′-deoxyuridine (BrdU) has turned out to be a versatile and reliable procedure. Labeling of the cells can be easily performed in vivo by feeding BrdU in the drinking water for extended periods of time or by an ip injection o

CLAIM

委屈

Adrenal-Glands

Fingerprint Analysis and Representation,e-specific, and inducible manner. This chapter provides a set of updated protocols that will help the reader to construct a vector for conditional gene targeting, to in vitro manipulate embryonic stem (ES) cells and to rapidly identify successfully targeted ES colonies. It also provides an updated l