Titlebook: B Cell Protocols; Hua Gu,Klaus Rajewsky Book 2004 Humana Press 2004

concubine

愤慨一下

Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice,mice expressing HEL as a systemic, intracellular, membrane-bound self-antigen. The same principles and methods apply to immunoglobulin transgenes. We briefly discuss the techniques that could be used to explore mechanisms of tolerance to systemic intracellular antigens in these mice.

刻苦读书

echnic

Verifying Floating-Point Algorithmsid rafts from B lymphocytes is described. Particular emphasis is given to the potential artifacts inherent in current procedures that rely on detergents to isolate lipid rafts and alternative technologies that may circumvent these.

Cultivate

miracle

Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells,rodissection. From these NP-specific recombined VDJ genes are amplified with family-specific primers by polymerase chain reaction (PCR) for deoxyribonucleic acid (DNA) sequencing. The methods described can be used to characterize origins and lifespan of plasma cells in vivo.

delegate

发电机

Encoding

Synthetic Fingerprint Generation,-cell stages. It also outlines assays for the characterization of these cells, including procedures for testing functional lineage restriction, determination of rearrangement status, analyses of gene expression at the ribonucleic acid (RNA) and protein level, and assessment of cell cycle state.

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