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Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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https://doi.org/10.1007/978-1-4614-7542-2sport in vitro. We outline protocols for labeling neurotrophins and tracking neurotrophin transport in these neurons. Our protocols can also be used to study axonal transport in other types of primary neurons such as cortical and hippocampal neurons.
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Acidosis and the Pulmonary Circulationre we describe a two-layered microfluidics chamber, engineered to allow for the culture of adult sensory neurons. This device was designed to promote the proper placement of adult sensory neurons in the somatic chamber in proximity of the microgrooves. We detail the production of the master mold, ho
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1064-3745 l Forebrain Cholinergic Neurons,    Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neu978-1-0716-1992-6978-1-0716-1990-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Imaging Axonal Transport in Ex Vivo Central and Peripheral Nervesed out in the context of a developed nerve containing both axons and glia. Here we outline how to image axonal transport ex vivo in sciatic and optic nerves, and the fimbria of the fornix. These methods could be altered to image other fluorescently labeled molecules, as well as different mechanisms of intracellular transport.
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Josh W. DiGiacomo,Daniele M. Gilkesnto thalamic nuclei of mice, thereby transducing neurons that project to the surface of the brain. Axonal projections and trafficking of organelles can be imaged with a 2-photon microscope through a chronically implanted window in the mouse skull in anesthetized as well as awake mice.
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