Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)anganese (MnII), gives a hyperintense signal in T. weighted MRI that can serve as a marker for axonal transport. Mn(II) enters active neurons via voltage-gated calcium channels and is transported via microtubule motors down their axons by fast axonal transport. Clearance of Mn(II) is slow. Scanning

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Imaging Diversity in Slow Axonal Transport proteins. These proteins typically move in the fast component of axonal transport and appear as “punctate structures” along axons. However, a large majority of transported proteins that move in the slow component of transport, typically show a “uniform diffusive glow” along axons when tagged to con

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Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neuronssport in vitro. We outline protocols for labeling neurotrophins and tracking neurotrophin transport in these neurons. Our protocols can also be used to study axonal transport in other types of primary neurons such as cortical and hippocampal neurons.

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Use of Microfluidics Chambers to Image Axonal transport in Adult Sensory Neuronsre we describe a two-layered microfluidics chamber, engineered to allow for the culture of adult sensory neurons. This device was designed to promote the proper placement of adult sensory neurons in the somatic chamber in proximity of the microgrooves. We detail the production of the master mold, ho

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Book 2022ging of RNA Transport and Translation in .Xenopus. Retinal Axons,    Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons,    Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neu

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