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Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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发表于 2025-3-21 18:22:49 | 显示全部楼层 |阅读模式
期刊全称Axonal Transport
期刊简称Methods and Protocol
影响因子2023Alessio Vagnoni
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Axonal Transport; Methods and Protocol Alessio Vagnoni Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t
影响因子.This volume covers a wide range of model systems from invertebrate to humans and .in vitro .and .in vivo..  Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.. ..Authoritative and cutting-edge, .Axonal Transport: Methods and Protocols .aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron..The chapters “ Live imaging of RNA Transport and Translation in .Xenopus. Retinal Axons,    Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons,    Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neu
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Cancer Drug Discovery and Developmentaptic strength. This transport is impaired in several neurodegenerative disorders such as Alzheimer’s disease. Thus, it is critical to understand the regulation and underlying mechanisms of the transport process. . provides a powerful experimental system for studying the interplay between synaptic a
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Cancer Drug Discovery and Developmentdge of the molecular mechanisms underlying axonal mRNA translation has rapidly expanded. However, tools to study mRNA localization and translation in real time with high spatial precision were not available until recently. Here, we present a live imaging approach to examine axonal mRNA trafficking a
发表于 2025-3-22 06:27:59 | 显示全部楼层
Hypoxia and Reactive Oxygen Specieser to remain healthy. This process, known as axonal transport, can be imaged in real time through epifluorescent imaging of fluorescently labeled proteins, organelles, and other cargoes. While this is most conveniently done in primary neuronal cultures, it is more physiologically relevant when carri
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Hypoxia in the Northern Gulf of Mexicol and functional integrity. Cargoes are transported along the microtubule (MT) network whose post-translational modifications influence transport dynamics. Here, we describe methods to modulate MT acetylation and record its impact on axonal transport in cultured mouse cortical projection neurons as
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