Titlebook: Axon Regeneration; Methods and Protocol Ava J. Udvadia,James B. Antczak Book 2023 The Editor(s) (if applicable) and The Author(s), under ex

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,Koyré, Cassirer and the History of Science,lesions, as well as after lesions that selectively target specific neuronal cell populations. An advantage of chemical retinal lesion for studying the process of regeneration is that the lesion is topographically widespread. This results in the loss of visual function as well as a regenerative respo

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Hypothesis Generation by Difference,d to severe spinal cord injury by forming a glial scar, which prevents further damage but also inhibits any regenerative growth, resulting in loss of function caudal to the injury site. The axolotl has become a popular system to elucidate the underlying cellular and molecular events that contribute

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Hypothesis Generation and Interpretationdeath. Experimentally injuring an axon makes it possible to study degeneration of the distal stump that was detached from the cell body and document the successive steps of regeneration. Precise injury reduces damage to the environment surrounding an axon, and thereby the involvement of extrinsic pr

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https://doi.org/10.1007/978-3-658-27588-4accessibility of DNA regulatory elements such as promoters and enhancers over the course of regeneration. This chapter describes methods for preparing ATAC-seq libraries from isolated zebrafish retinal ganglion cells (RGCs) following optic nerve crush at selected post-injury time points. These metho

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Ava J. Udvadia,James B. AntczakIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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