Titlebook: Axon Regeneration; Methods and Protocol Ava J. Udvadia,James B. Antczak Book 2023 The Editor(s) (if applicable) and The Author(s), under ex

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Surgical Methods in Postmetamorphic ,: Optic Nerve Crush Injury Model,ferent cell types, retinal ganglion cells (RGCs) are the only cell type connecting the eye to the brain. Optic nerve crush injuries, wherein RGC axons are damaged without severing the optic nerve sheath, can serve as a model for traumatic optical neuropathies as well as some progressive neuropathies

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A Reproducible Spinal Cord Crush Injury in the Regeneration-Permissive Axolotl,d to severe spinal cord injury by forming a glial scar, which prevents further damage but also inhibits any regenerative growth, resulting in loss of function caudal to the injury site. The axolotl has become a popular system to elucidate the underlying cellular and molecular events that contribute

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Profiling Dynamic Changes in DNA Accessibility During Axon Regeneration After Optic Nerve Crush in accessibility of DNA regulatory elements such as promoters and enhancers over the course of regeneration. This chapter describes methods for preparing ATAC-seq libraries from isolated zebrafish retinal ganglion cells (RGCs) following optic nerve crush at selected post-injury time points. These metho

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978-1-0716-3014-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines