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Titlebook: Axon Regeneration; Methods and Protocol Ava J. Udvadia,James B. Antczak Book 2023 The Editor(s) (if applicable) and The Author(s), under ex

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发表于 2025-3-21 17:26:35 | 显示全部楼层 |阅读模式
期刊全称Axon Regeneration
期刊简称Methods and Protocol
影响因子2023Ava J. Udvadia,James B. Antczak
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Axon Regeneration; Methods and Protocol Ava J. Udvadia,James B. Antczak Book 2023 The Editor(s) (if applicable) and The Author(s), under ex
影响因子.This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both .in vivo .and .in vitro. approaches, physical injury models, methods for the isolation/analysis of various macromolecules, live and fixed imaging of regenerating axons, and for quantifying behavioral endpoints enable measurements of regenerative success. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .Axon Regeneration: Methods and Protocols. aims to be comprehensive guide for researchers..
Pindex Book 2023
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发表于 2025-3-21 21:35:17 | 显示全部楼层
Defining Selective Neuronal Resilience and Identifying Targets of Neuroprotection and Axon Regeneraur goal is to identify differences in the dynamics of survival among 46 molecularly defined RGC types together with molecular signatures that correlate with these differences. The data consists of scRNA-seq profiles of RGCs collected at six time points following optic nerve crush (ONC) (see companio
发表于 2025-3-22 03:26:50 | 显示全部楼层
Retinal Ganglion Cell Axon Fractionation,. Here we present a fractionation method to isolate regenerating RGC axons for downstream analysis using immunomagnetic separation of cholera toxin subunit B (CTB)-bound RGC axons. After optic nerve tissue dissection and dissociation, conjugated CTB is used to bind preferentially to regenerated RGC
发表于 2025-3-22 05:03:53 | 显示全部楼层
Analysis of Immediate Early Gene Expression Levels to Interrogate Changes in Cortical Neuronal Actie research. Due to straightforward detection methods such as in situ hybridization and immunohistochemistry, changes in IEG expression can be easily visualized across brain regions and in response to physiological and pathological stimulation. Based on in-house experience and existing literature, .
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发表于 2025-3-22 15:25:07 | 显示全部楼层
Transneuronal Delivery of Cytokines to Stimulate Mammalian Spinal Cord Regeneration,in regenerating after injury. Many of these key fiber tracts originate from deep brain stem nuclei that are difficult to access. Here we detail a new methodology that achieves functional regeneration in mice after a complete spinal cord crush, describing the crushing procedure itself, intracortical
发表于 2025-3-22 18:32:53 | 显示全部楼层
Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury,e genome-wide techniques that provide information relative to gene expression, chromatin binding sites, and chromatin accessibility, respectively. Here we describe RNA-seq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq, and ATAC-seq in dorsal root ganglia (DRG) after sciatic nerve or dorsal column axotomy,
发表于 2025-3-22 22:09:38 | 显示全部楼层
发表于 2025-3-23 04:34:52 | 显示全部楼层
Analysis of Axonal Regrowth and Dendritic Remodeling After Optic Nerve Crush in Adult Zebrafish, contrast to mammals, adult zebrafish show a robust regeneration capacity after CNS injury and form the ideal model organism to further unravel the underlying mechanisms for both axonal and dendritic regrowth upon CNS damage. Here, we first describe an optic nerve crush injury model in adult zebrafi
发表于 2025-3-23 08:11:56 | 显示全部楼层
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