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Titlebook: Awake Behaving Mesoscopic Brain Imaging; James E. Niemeyer,Hongtao Ma,Theodore H. Schwartz Book 2025 The Editor(s) (if applicable) and The

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Jonghwa Kim,Mincheol Whang,Jincheol Woo further investigate the relationship among different cortex regions. Here, we present an overview of the principle, development, and application of GENIs, focusing on their joint utilization with mesoscopic imaging.
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Luís Duarte,Marco de Sá,Luís Carriçonal detection, and discuss the challenges and opportunities for large-scale imaging. The chapter aims to provide readers with an overview of the RUSH system and its potential for advancing system biology studies.
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Mesoscopic Imaging of Neurotransmitters and Neuromodulators with Genetically Encoded Sensors further investigate the relationship among different cortex regions. Here, we present an overview of the principle, development, and application of GENIs, focusing on their joint utilization with mesoscopic imaging.
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Wide-Field Calcium Imaging of Mesoscale Networks Underlying the Encoding of Skilled Voluntary Movemeation of the optical window, the methods to motivate the animal to learn and perform the RtG task in the behavioral platform, and the image processing and data analysis of the behavioral data and the fluorescent calcium image will be described.
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Widefield Imaging Combined with a Transparent Electrographic Probebe (Neuro-FITM). This multimodal approach allows simultaneous recording of neural activity from the dorsal cortex and subcortical brain regions in awake, head-fixed mice. This protocol provides guidelines on how to implement this technique, including surgical preparation, recording setup, and data processing.
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Mesoscale Imaging of Neural Dynamics in Epilepsyhods and experimental techniques that can be employed to study seizures in awake behaving rodents, with a particular focus on simultaneously applying genetically encoded calcium indicators and hemodynamic imaging. These methods can be easily extended to normal brain mechanisms in healthy brain as well as other neurological diseases.
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