Titlebook: Awake Behaving Mesoscopic Brain Imaging; James E. Niemeyer,Hongtao Ma,Theodore H. Schwartz Book 2025 The Editor(s) (if applicable) and The

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Mesoscale Ca++ Imaging of Seizures and Interictal Activity in Models of Chronic Epilepsyelate features of interictal abnormalities to seizures, and (4) evaluate the efficacy of therapies in normalizing cortical activity. The low cost, usefulness, and flexibility of the approach should encourage those investigating animal models of epilepsy and other neurological diseases to adopt it as part of their phenotyping repertoire.

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Book 2025ers in this book are organized into four parts: Part One highlights novel imaging modalities, such as neurotransmitter and single-cell resolution imaging and analyses of neuroimaging data in awake rodents. Part Two covers technical innovations such as head-mounted camera systems, multi-site photomet

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Mesoscopic Imaging of Neurotransmitters and Neuromodulators with Genetically Encoded Sensorsmission mechanisms of NTs or NMs (NTs/NMs), it is critical to monitor the dynamics of NTs/NMs with high sensitivity, specificity, and spatiotemporal resolution. With recent advances in genetically encoded NT/NM indicators (GENIs) and mesoscopic imaging, it is now possible to visualize the spatiotemp

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Real-Time Ultra-Large-Scale Imaging with High-Resolution Microscopyof biological systems with unprecedented space–bandwidth product and data throughput. We describe the design and implementation of the RUSH system, which comprises a customized objective lens, a camera array, and a computer cluster. We also demonstrate the applications of the RUSH system in imaging

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Mesoscale Calcium (Ca2+) Imaging in Freely Behaving Miceously during awake behavior. While much of the work in the field has focused on studying cortical dynamics during head-fixed preparations, we have recently developed the mini-mScope—a head mounted widefield imaging microscope capable of imaging large swathes of the dorsal cortex of freely behaving m

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High-Density Multichannel Fiber Photometryelopment of genetically encoded calcium indicators has advanced experimental approaches in systems neuroscience to assess brain activity simultaneously in multiple brain regions. Here, we provide a brief overview of optical recording techniques at this mesoscale level and of their combination with g

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Widefield Imaging Combined with a Transparent Electrographic Probedvances in recording techniques have brought about increased knowledge of how different brain regions cooperate in various cognitive processes. Here, we describe a protocol combining widefield calcium imaging with electrical recordings using a flexible, insertable, and transparent microelectrode pro

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