Titlebook: Autophagy and Cancer; Methods and Protocol Helin Norberg,Erik Norberg Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

cloture

Strings and Fundamental Interactions,cribe a new tool for the qualitative and quantitative determination of autophagic flux using a dual lentiviral reporter system that generates a fusion HiBiT-GFP-LC3B protein suitable for generating stable cell lines.

欢腾

定点

Stefano Braccini,Francesco Fidecarodamaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.

量被毁坏

者变

A Dual HiBiT-GFP-LC3 Lentiviral Reporter for Autophagy Flux Assessmentcribe a new tool for the qualitative and quantitative determination of autophagic flux using a dual lentiviral reporter system that generates a fusion HiBiT-GFP-LC3B protein suitable for generating stable cell lines.

extinct

Inordinate

Induction and Detection of Mitophagydamaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.

Ornithologist

Book 2022includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.. .Authoritative and cutting-edge, .Autophagy and Cancer: Methods and Protocols .aims to be a useful practical guide to researches to help further their study in this field. .

灰姑娘

机制

Isolation of Autophagy Competent Lysosomes from Cancer Cells by Differential Large-Scale Multilayerel changes by comparative quantitative measurement. This method has been used to enrich lysosomes from a variety of cancer cells with activated chaperone-mediated autophagy, but can be optimized for other cell lines and tissues for multiple autophagy-induced conditions.