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Titlebook: Autophagy and Cancer; Methods and Protocol Helin Norberg,Erik Norberg Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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期刊全称Autophagy and Cancer
期刊简称Methods and Protocol
影响因子2023Helin Norberg,Erik Norberg
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Autophagy and Cancer; Methods and Protocol Helin Norberg,Erik Norberg Book 2022 The Editor(s) (if applicable) and The Author(s), under excl
影响因子.This volume includes comprehensive methods and protocols on autophagic pathways in cancer disorders. Chapters cover basic principles of autophagy methodology in cancer cells, translational approaches, systematic computational analyses of TCGA (.The Cancer Genome Atlas.). .cohorts, .CCLE .(.The Cancer Cell Line Encyclopedia) analysis, CRISPR genetics, metabolomics, biochemistry, and immunohistochemistry methodologies. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.. .Authoritative and cutting-edge, .Autophagy and Cancer: Methods and Protocols .aims to be a useful practical guide to researches to help further their study in this field. .
Pindex Book 2022
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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/166837.jpg
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978-1-0716-2073-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Gravity, Particles, and Astrophysicslysosomal content, allowing specific lysosomal investigations induced by autophagy. In this protocol chapter, we describe detailed practical instructions and advices for an efficacious lysosomal enrichment and isolation procedure by differential multilayered density gradient centrifugations using hu
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,1905–1930: The Golden Age of Physics,discovered for autophagy. At the same time, precise monitoring of autophagy has become important, and western blotting and fluorescence microscopy of the marker protein LC3 is widely used for this purpose. Here, we describe a modification of the widely used method, number of LC3 dots per cell. This
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