Titlebook: Autophagy and Cancer; Methods and Protocol Helin Norberg,Erik Norberg Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Concepts and Design of Introducing Synthetic MicroRNAs into Mammalian Cellsd methodological steps to introduce synthetic microRNA drugs into target cells in vitro and in vivo and how to monitor their function. In addition, it provides insights on how to control the adverse effects when ectopically expressing synthetic microRNA mimic molecules.

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A Balance Between Autophagy and Other Cell Death Modalities in Cancerophagy-related pathologies gave rise to the development of new therapeutic drugs. Here, we summarize the current knowledge on the autophagic role in disease pathogenesis, particularly in cancer, and the interplay between autophagy and other cell death modalities in order to combat cancer.

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,1905–1930: The Golden Age of Physics,the marker protein LC3 is widely used for this purpose. Here, we describe a modification of the widely used method, number of LC3 dots per cell. This protocol provides the proportion of vesicular LC3 staining over the total LC3 staining in the same cell. The approach is well suitable for quantification of endogenous LC3.