Titlebook: Autoantibodies; Methods and Protocol Gunnar Houen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 etiology.aut

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Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry,bodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.

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Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay, assay of determination of TGA was newly developed using electrochemiluminescence (ECL) technology. This ECL assay has been demonstrated to be more sensitive than current standard radio-binding assay (RBA) in detecting TGA and can detect TGA earlier among high-risk young children followed from birth.

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https://doi.org/10.1007/978-1-4939-8949-2etiology; autoimmunity; Autoantibody structures; T cells; Golgi apparatus; Immunology; antibodies

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Autoantibodies978-1-4939-8949-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-8348-9005-4 assay of determination of TGA was newly developed using electrochemiluminescence (ECL) technology. This ECL assay has been demonstrated to be more sensitive than current standard radio-binding assay (RBA) in detecting TGA and can detect TGA earlier among high-risk young children followed from birth.

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