Titlebook: Autoantibodies; Methods and Protocol Gunnar Houen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 etiology.aut

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Determination of Autoantibodies to Salivary Gland Antigens,ognostic applications. In this chapter, we describe the procedures and protocols for determining autoantibodies to salivary gland antigens with proteomics-related methods, including 2D gel electrophoresis, liquid chromatography–tandem mass spectrometry (LC-MS/MS), Western blotting, protein microarray, and enzyme-linked immunosorbent assay (ELISA).

pineal-gland

Measurement of Autoantibodies to Gastric H+,K+-ATPase (ATP4A/B) Using a Luciferase Immunoprecipitatagged recombinant ATP4A and ATP4B, their immunoprecipitation with test sera, the recovery and washing of immune-complexes with a protein-A coated resin, and the quantification of autoantibodies by addition of a luciferase substrate and the measurement of the light output from captured luciferase-tagged antigens.

sparse

Analysis of C3 Nephritic Factors by ELISA,the diagnostic workup of C3 glomerulopathy, but their identification is difficult presumably due to considerable heterogeneity. Therefore it is recommended to use a combination of different analysis methods for the detection of C3NeF. Here we present an ELISA method for detection of C3NeF.

植物学

Anti-factor H Autoantibodies Assay by ELISA,ical management of the patients..Anti-Factor H antibodies are detected and quantified by an ELISA assay. The aim of this chapter is to describe the procedure to determine anti-FH autoantibodies and to provide information about their biological significance.

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