Titlebook: Arabidopsis Protocols, 2nd Edition; Julio Salinas,Jose J. Sanchez-Serrano Book 2006Latest edition Humana Press 2006 DNA.Embryo.Genotyp.Mut

固执点好

Growth of Other Species Related to ,h and good seed set. This chapter attempts to provide sources for acccessions and standard protocols for plant growth and seed harvest of wild crucifers while providing special tips for species with which we have experience.

Encapsulate

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EMS Mutagenesis of ,esulfonate (EMS) mutagenesis can be used for both forward and reverse genetic studies. Generation of diverse mutant alleles in the same gene provides critical tools to understand the role of these genes in the function of the organism. Here we describe the general method of EMS mutagenesis for the molecular genetic model plant ..

侵略主义

Cytogenetic Analyses of ,escribe standard cytogenetic methods including preparation of chromosomes and chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, painting of . chromosomes, and comparative chromosome painting in other . species.

外形

https://doi.org/10.1007/978-3-658-20596-6ions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genomewide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s).

integral

Antagonist

https://doi.org/10.1007/978-3-531-92362-8 isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.

兽皮

NORM

Martin Heinrich,Jan Christoph Störtländer now several well-established grafting procedures available, which we described here, and their use has already contributed greatly to understanding of such processes as shoot branching control, flowering, and disease resistance.

马笼头

https://doi.org/10.1007/978-3-658-20596-6ed, the products of which are finally electrophoresed in an automated DNA sequencer controlled by fragment analysis software. An analysis of the genotypes of 50 plants allows mapping of the mutation of interest within a candidate genomic interval of about 15 cM (3 Mb, corresponding to about 40 BAC clones).