Titlebook: Arabidopsis Protocols, 2nd Edition; Julio Salinas,Jose J. Sanchez-Serrano Book 2006Latest edition Humana Press 2006 DNA.Embryo.Genotyp.Mut

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ATP861

Prevention and Control of Pests and Diseasesthe plants outright, insect pests and microbial pathogens can cause subtle changes in gene expression or plant metabolism that affect experimental results. Therefore, regular scouting for infestations, frequent cleaning of plant growth areas, proper disposal of dead or diseased plant material, and c

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Growth of Other Species Related to ,otocols for growth and maintenance of these plant species. Like ., many wild crucifers are amenable to growth in greenhouses, growth rooms, and growth chambers for laboratory study. Some aspects of their care are quite similar to .. However, several unique traits, such as perennial growth habit, lon

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Genetic Analysisis chapter. These include the determination of the number of genes involved in the observed trait’s variation, the determination of dominance relationships between alleles of the same locus, and epistatic interactions with related genetic variants. A new variant should be compared with previously id

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QTL Analysisal, and evolutionary levels. A major step in these studies is the identification and location of quantitative trait loci (QTLs) determining the variation for trait(s) of interest, so-called QTL analysis. Here we provide general methods to achieve this goal, including (1) the selection of wild access

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EMS Mutagenesis of ,esponses. Various approaches for mutagenesis involving chemical, irradiation, and insertional methods have been developed; each has advantages and disadvantages for the study of gene function. In this post-genomic era, the use of reverse genetic approaches to understanding the role of genes in growt

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Low-Resolution Mapping of Untagged Mutationsy labeled oligonucleotides as primers. For the genotyping of a single plant from a mapping population, only two simultaneous amplifications are required, the products of which are finally electrophoresed in an automated DNA sequencer controlled by fragment analysis software. An analysis of the genot

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