Titlebook: Applications of Genome Modulation and Editing; Paul John Verma,Huseyin Sumer,Jun Liu Book 2022 The Editor(s) (if applicable) and The Autho

STALE

Aboriginal Peoples Survey, Canada (APS)nomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest . (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.

frugal

Efficient Generation of Stable Cell Lines with Inducible Neuronal Transgene Expression Using the pigngle isothermal reaction. We demonstrate an application of this approach to generate a stably transfected pluripotent stem cell line that can be induced to express a transcription factor transgene and rapidly differentiate into neurons in a single step.

GONG

CRISPR/Cas9 Mutagenesis to Generate Novel Traits in , for Sterile Insect Techniquenomic modification of non-model organisms. Here, we describe the manual microinjection of CRISPR/Cas9 reagents into tephritid pest . (Queensland fruit fly) embryos to introduce ideal traits as well as the molecular methods used to detect successful mutagenesis.

发微光

CRISPR-on for Endogenous Activation of , Expression in Bovine Embryosapplied science, such as cell reprogramming and cell fate differentiation for regenerative medicine..In this chapter, we describe the materials and methods of the CRISPR-on system for activation of the endogenous SMARCA4 expression in bovine embryos

闯入

CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowflytailed guide for using the . Cas9 RNA-guided nuclease to efficiently generate both knockout and knock-in mutations in screwworm and sheep blowfly. These methods should accelerate genetic investigations in these and other closely related species and lead to a better understanding of the roles of selected genes in blowfly development and behavior.

自负的人

Targeted Gene Editing in Porcine Germ Cellsrmline genetic modification through gene edited spermatogonia provides an effective alternative to how these models are developed. Here, we report an improved technique of gene editing in spermatogonia of pigs using CRISPR-Cas9 to generate different edits that reflect the genotypes of human diseases.

Ingenuity

Book 2022by-step, readily reproducible protocols. ..Authoritative and cutting-edge, .Applications of Genome Modulation and Editing .aims to be a useful and practical guide for researchers to commence or advance their study in this field. .

Bouquet

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经典

Absolute Individual Deprivation in Europeair (HDR) in mouse zygotes. The mouse model carrying the identical . mutation in slick cattle may provide a useful animal model to study the pathway of thermoregulation and the mechanism of heat-tolerance in the livestock.