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Titlebook: Applications of Genome Modulation and Editing; Paul John Verma,Huseyin Sumer,Jun Liu Book 2022 The Editor(s) (if applicable) and The Autho

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发表于 2025-3-21 16:42:35 | 显示全部楼层 |阅读模式
期刊全称Applications of Genome Modulation and Editing
影响因子2023Paul John Verma,Huseyin Sumer,Jun Liu
视频videohttp://file.papertrans.cn/160/159447/159447.mp4
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Applications of Genome Modulation and Editing;  Paul John Verma,Huseyin Sumer,Jun Liu Book 2022 The Editor(s) (if applicable) and The Autho
影响因子.This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. ..Authoritative and cutting-edge, .Applications of Genome Modulation and Editing .aims to be a useful and practical guide for researchers to commence or advance their study in this field. .
Pindex Book 2022
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978-1-0716-2303-9The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Gérard Duhaime,Sébastien Lévesqueected into mouse embryos at different preimplantation stages. Subsequently, simple DNA plasmids were used to microinject into the pronuclei of fertilized mouse oocytes and that method became the reference for many years. The isolation of embryonic stem cells together with advances in genetics allowe
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Gérard Duhaime,Sébastien Lévesquection expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to
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https://doi.org/10.1007/978-3-031-17299-1 the biotechnology industry. In this chapter, we describe an easy to establish, robust, and practical pipeline that can be used to generate immortalized cell lines, from different tissues, to capture cell linage context and validate the tools required for genome editing and genetic modification. Thi
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