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Titlebook: Antimicrobial Peptides; Methods and Protocol Andrea Giuliani,Andrea C. Rinaldi Book 2010 Springer Science+Business Media, LLC 2010 Escheric

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Expression and Purification of Recombinant α-Defensins and α-Defensin Precursors in ,es of C18 RP-HPLC purified peptides are confirmed by MALDI-TOF mass spectrometry, and peptide homogeneity is assessed using analytical RP-HPLC and acid-urea polyacrylamide gel electrophoresis. α-Defensins prepared in this manner are biochemically equivalent to the natural molecules.
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Non-Destructive Evaluation Seriesdes. We used various HPLC techniques, combined with antimicrobial testing as read-out system. In parallel, SDS-PAGE analyses as well as electrospray ionization mass spectrometry were used for further biochemical characterization as well as purity assessment.
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Tribological and Contact Area Effects,hted here – a mix of antimicrobial, LPS-sequestering/neutralization, and immunomodulatory features make cationic peptides, and especially synthetic or semi-synthetic amphiphilic compounds built on their scheme, attractive candidates for novel drugs to be administered in antisepsis therapies. These t
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Solutions in spherical coordinates, discussed. Solid-state NMR spectra of both lipids and peptides have been recorded, and through the ensemble of measurements a detailed picture of these complex peptide–lipid supramolecular systems has finally emerged.
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