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Titlebook: Antimicrobial Peptides; Methods and Protocol Andrea Giuliani,Andrea C. Rinaldi Book 2010 Springer Science+Business Media, LLC 2010 Escheric

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期刊全称Antimicrobial Peptides
期刊简称Methods and Protocol
影响因子2023Andrea Giuliani,Andrea C. Rinaldi
视频video
发行地址Includes contributions from scientists from different disciplines and backgrounds, from both industry and academia.Provides notes sections describing major sources of problems that may occur with a pa
学科分类Methods in Molecular Biology
图书封面Titlebook: Antimicrobial Peptides; Methods and Protocol Andrea Giuliani,Andrea C. Rinaldi Book 2010 Springer Science+Business Media, LLC 2010 Escheric
影响因子.The action of antimicrobial peptides (AMPs), ranging from direct killing of invading pathogens to immune response modulation and other complex biological responses, has stimulated research and clinical interest for more than two decades, but the area is still burgeoning due to emerging discoveries in the functions, roles, and regulation of AMPs, thus making the study of antimicrobial peptides a multi-disciplinary and rapidly evolving field. In .Antimicrobial Peptides: Methods and Protocols., leading investigators present a broad, up-to-date collection of current research and experimental methods for the isolation, characterization, production, and optimization of antimicrobial peptides. Additional chapters detail methodologies in several microscopy techniques, high-throughput screening, QSAR modeling, and computer-aided design used to study these compounds, while key review articles survey potential medical applications of antimicrobial peptides as innovative anti-infective and immunomodulatory agents, as well as emerging discoveries in their function, regulation, and roles in innate immunity. As a volume in the highly successful .Methods in Molecular Biology.™ series, chapters in
Pindex Book 2010
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Strategies for the Isolation and Characterization of Antibacterial Lantibioticssistant strains. We describe a bioassay-based method suitable for finding antibacterial lantibiotics from actinomycete strains and provide selected procedures for characterizing newly discovered lantibiotics for their antibacterial properties.
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Production of Recombinant Antimicrobial Peptides in Bacteriacrobial peptides into inclusion bodies, which is affective for the production of large quantities of antimicrobial peptides. Chemical reagents for cleaving peptide bond between antimicrobial peptides and fusion proteins such as cyanogen bromide and diluted acid are selective and provide antimicrobial peptides for biological studies in short time.
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Binding and Permeabilization of Model Membranes by Amphipathic Peptidesf time, rather than in steady state. The activity of these amphipathic peptides toward model membranes is usually measured by dye efflux kinetics. One of those methods, based on self-quenching of carboxyfluorescein, is described here, together with a discussion of caveats and pitfalls of the corresponding analysis and interpretation.
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High-Throughput Screening for Antimicrobial Peptides Using the SPOT Technique. H1001) in which a luminescence-encoding gene cassette has been introduced. The intensity of light produced is directly dependent on the energy level of the bacteria. This screening supports the development of new drugs against multidrug-resistant bacteria.
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