Titlebook: Antimicrobial Peptides; Methods and Protocol Andrea Giuliani,Andrea C. Rinaldi Book 2010 Springer Science+Business Media, LLC 2010 Escheric

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https://doi.org/10.1007/978-3-030-39543-8r targets. Here, we describe in detail two biophysical techniques that can be used to measure the membrane activities of AMPs and antimicrobial peptidomimetics: (1) a calcein leakage assay to investigate interactions between AMPs/peptidomimetics with large unilamellar vesicles and (2) a potential-se

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https://doi.org/10.1007/978-3-030-39543-8e involved, e.g., in cellular fusion or host defense, where they can cause not only merging but also the destabilization of cell membranes. Many factors determine how these typically amphipathic peptides interact with the lipid bilayer. For example, the peptide orientation in the membrane determines

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https://doi.org/10.1007/978-3-031-44002-1 action is urgently needed. In this context, cationic antimicrobial peptides (AMPs) are considered promising candidates. Although there is evidence that, in contrast to conventional antibiotics, microbial membranes are the principal target of a large number of AMPs, thus making it difficult for the

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Superconductors and Magnetic Materials,iological phenomena. From all-atom MD simulations, we are now able to visualize in detail the interactions between antimicrobial peptides (AMPs) and a variety of membrane mimics. This helps us to understand the molecular mechanisms of antimicrobial activity and toxicity. This chapter describes how t

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1064-3745 and immunomodulatory agents, as well as emerging discoveries in their function, regulation, and roles in innate immunity. As a volume in the highly successful .Methods in Molecular Biology.™ series, chapters in978-1-4939-6128-3978-1-60761-594-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Purification of Antimicrobial Peptides from Human Skindes. We used various HPLC techniques, combined with antimicrobial testing as read-out system. In parallel, SDS-PAGE analyses as well as electrospray ionization mass spectrometry were used for further biochemical characterization as well as purity assessment.