Titlebook: Antigen Processing; Methods and Protocol Peter van Endert Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na

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In Vitro Studies of MHC Class I Peptide Loading and Exchange,molecular level how antigenic peptides are selected for presentation to CD8+ T-cells. Here, we describe practical procedures for generating a cell-free system made up of MHC class I molecules and tapasin that can be used for mechanistic studies of peptide loading and exchange.

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F. Lamy,D. Gibson,M. Ledoux,J. C. Moreuxely, the numerous advantages of poxvirus recombinants have made the Vaccinia expression system a mainstay in the study of processing and presentation over the past two decades. In an attempt to address one shortcoming of Vaccinia virus while simultaneously retaining the benefits inherent to poxvirus

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1064-3745 ing known pitfalls.. .Cutting-edge and comprehensive, .Antigen Processing: Methods and Protocols, Second Edition. is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field..978-1-4939-9452-6978-1-4939-9450-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies,icity for peptide–MHC complexes are created, this method will be helpful in quantifying the exact numbers of complexes generated by cell types and relating these numbers to physiological outcomes of T cell activation.

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A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processir MHC I downregulation has been identified, the same cells can be used to elucidate the mechanism of action. The stage at which interference with antigen processing occurs can be identified using specific assays. An essential step frequently targeted by viruses is the translocation of peptides into

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