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Titlebook: Antigen Processing; Methods and Protocol Peter van Endert Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na

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发表于 2025-3-21 17:34:22 | 显示全部楼层 |阅读模式
期刊全称Antigen Processing
期刊简称Methods and Protocol
影响因子2023Peter van Endert
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Antigen Processing; Methods and Protocol Peter van Endert Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na
影响因子The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Cutting-edge and comprehensive, .Antigen Processing: Methods and Protocols, Second Edition. is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field..
Pindex Book 2019Latest edition
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发表于 2025-3-21 22:24:06 | 显示全部楼层
https://doi.org/10.1007/978-3-319-40832-3 in the analysis of MHC class I molecule biosynthesis and degradation as well as complementary studies using conventional conformationally specific antibodies. Coupled together with pharmacological manipulation which can target both biosynthetic and degradative pathways, this offers a powerful tool in analyzing MHC class I molecules.
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Trimming of MHC Class I Ligands by ERAP Aminopeptidases,function of ERAPs in peptide repertoire development. Specifically, our assay allows for monitoring trimming of free but also of MHC I-bound peptides which may reflect the physiological situation best.
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Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs,ple, the ligands of monoallelic C1R cells and multiallelic peripheral blood mononuclear cell tissue will be identified and a scoring matrix for the prediction of HLA-C*01:02-presented T cell epitopes will be developed.
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https://doi.org/10.1007/978-3-319-40832-3tionated using reverse phase high performance liquid chromatography and detected by the activation of CD8+ T cell hybridomas. The results provide information on the structure and amount of the peptides and yield insights into the mechanisms that generate the naturally processed peptides.
发表于 2025-3-23 06:00:03 | 显示全部楼层
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