Titlebook: Antigen Processing; Methods and Protocol Peter van Endert Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na

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Evaluating CD8+ T Cell Responses In Vitro,ology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8. T cell responses. Allowing for real-time evaluation of target cell viability, some of them are particularly attractive and are described here together with traditional assays.

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Monitoring MHC-II Endocytosis and Recycling Using Cell-Surface Protein Biotinylation-Based Assays,nt that contains a cleavable disulfide bond that allows for reversible labeling of proteins. Biotinylation is rapid and stable, and does not transfer from cell to cell, and the small size of the biotin probe does not affect cell function.

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