Titlebook: Antifungal Drug Resistance; Methods and Protocol Damian J. Krysan,W. Scott Moye-Rowley Book 2023 The Editor(s) (if applicable) and The Auth

Kinetic

https://doi.org/10.1007/BFb0117709 step in understanding how pathogenic yeast, such as . (i.e.., and others) as well as ., behave in response to antifungal therapeutics. Here we describe simple in vitro protocols for the quantification of drug responses with subpopulation resolution.

Prosaic

Elastic-Plastic Fracture Mechanics a small number of changes that can be evaluated for association with drug resistance. Here, we provide a detailed protocol for the computational analysis of genome sequences to identify variants associated with drug resistance.

鄙视

1064-3745 expertsThis detailed volume aims to elucidate the molecular mechanisms that underlie antifungal resistance. The book highlights methods to identify and characterize antifungal activity, to define and characterize strains with altered responses to antifungal drugs, to investigate the genetic and mol

annexation

https://doi.org/10.1007/BFb0117709phology of these important organisms. In this chapter, we describe a protocol for utilizing a bioluminescent . strain for identifying small molecules that potentiate the activity of the triazole antifungal drug fluconazole. The assay holds great promise for identifying small molecules with activity against filamentous fungal pathogens.

熔岩

Elastic-Plastic Fracture Mechanicsften identify compounds with no known mechanism of action. Herein, we describe a method utilizing the human fungal pathogen . to identify antifungal drug targets through the isolation of spontaneous resistant mutants, antifungal testing, whole-genome sequencing, and variant analysis.