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Titlebook: Antibody Engineering; Roland Kontermann,Stefan Dübel Book 20011st edition Springer-Verlag Berlin Heidelberg 2001 Amino acid.Antigen.Cell p

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期刊全称Antibody Engineering
影响因子2023Roland Kontermann,Stefan Dübel
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发行地址Comprehensive collection of proven lab protocols in the expanding field of antibody engineering.Beginners are introduced to the basic methods with detailed instructions.Professional antibody engineers
学科分类Springer Lab Manuals
图书封面Titlebook: Antibody Engineering;  Roland Kontermann,Stefan Dübel Book 20011st edition Springer-Verlag Berlin Heidelberg 2001 Amino acid.Antigen.Cell p
影响因子Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more..This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered.
Pindex Book 20011st edition
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Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly et al. 2000), very many hybridomas have been generated, and are continuously being made, which produce monoclonal antibodies with very interesting properties. Even when naive and synthetic libraries may be a source of antibodies, often the immune response of an experimental animal may be of interes
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Construction of scFv from Hybridoma by Two-Step Cloning available from hybridoma cell lines. Here, a method is presented to obtain the genetic information for the antigen binding part of the antibody from hybridoma cells, and to assemble it into a functional bacterially expressed fusion protein (scFv fragment). To achieve this, vectors have been constru
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Generation of Naive Human Antibody Librariesdes and folded proteins could be displayed on the surface of filamentous bacteriophage (Smith, 1985; Bass et al., 1990). Since the generation of the first human antibodies by phage display (Winter et al., 1994), the technology has developed to the point where large scFv repertoires have been created
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Generation of Antibody Gene Libraries from Seropositive Human Donors however, antibodies to threatening antigens cannot be produced, as in the case of most cancer cells, or they appear too late to control the antigen, as e. g. in the case of intoxication. In this situation, therapeutic antibodies can be applied to help our own immune system. Further, therapeutic ant
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Generation of Rabbit Immune Librariesat proteins provides an alternative strategy for the rapid generation of monoclonal antigen-binding proteins. Various antibody-displaying phage libraries have been described, which are based on the B cell repertoire of rearranged immunoglobulin genes from spleen and bone marrow of previously immuniz
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