Titlebook: Antibody Engineering; Roland Kontermann,Stefan Dübel Book 20011st edition Springer-Verlag Berlin Heidelberg 2001 Amino acid.Antigen.Cell p

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dendrites

,Opfer und ihre Rechte (§§ 65–70),le, an immense number of different molecules, bearing different specificities, is required. This diversity is generated by somatic recombination and hypermutagenesis of a set of variant genes. The genetic information for this repertoire of different antibodies is stored in the B-cell pool of our lym

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有恶臭

,Opfer und ihre Rechte (§§ 65–70),wever, there are a number of potential pitfalls in using V region PCR. Mutations within the 5′ or 3′ ends of the V genes may inhibit primer annealing and so prevent amplification. Another problem is the presence of other V genes within the hybridoma which are preferentially amplified. These arise fo

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Einführung in die neue Strafprozessordnungs displayed on the surface of filamentous phage. Phagemid vectors are used for placing randomly paired light (L) and heavy (H) chain coding regions under transcriptional control of . The L (or H) chain coding region is fused in-frame with the phage gene, .g., coding for a truncated version of the ph

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FLIT

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,Ausschließung und Befangenheit (§§ 43–47), from phage display libraries (Marks et al., 1991; Harrison et al., 1996; for review see Winter et al., 1994). The protocol shown here describes selections from a phagemid library (Vaughan et al., 1996) (see also Chapter 6) based on phagemid vectors such as pCANTAB6 (McCafferty et al., 1994). In the