Titlebook: Antibiotic Resistance Methods and Protocols; Stephen H. Gillespie Book 20011st edition The Editor(s) (if applicable) and The Author(s), un

Constitution

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Quantitative, Single-Tube, Nested, PCR (QSTN-PCR) for Determining the Antibiotic Susceptibility of ,ay be infected with this organism (.). A sensitive, PCRbased system to test mycobacterial antibiotic susceptibility is one approach to this problem. We reasoned that a quantitative PCR could detect the growth of bacilli by detecting an increase in the amount of mycobacterial DNA. Inclusion of an eff

Affluence

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Buttress

Processes

慎重

palliative-care

Quantification of M. tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosismear to negative, or culture positive sputum to negative. AFB enumeration lacks sensitivity and specificity and the culture of . (MTB) bacteria can take weeks. Quantitative estimates of MTB DNA in sputum have been shown to correlate with numbers of viable bacilli before the onset of chemotherapy (.-

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Isolation of , RNA from Sputumrticular mRNA transcripts expressed in . (MTB) bacilli under various conditions can provide insight into models of pathogenesis. MTB mRNA has also been demonstrated to correspond to viability in drug-treated MTB culture systems (.), thus providing an alternative to the lengthy and often difficult ba

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Detection of Viable , by Reverse Transcriptase-Strand Displacement Amplification of mRNA. Numerous systems have now been described for the amplification and detection of DNA or rRNA sequences that are specific for the . complex (.-.). While useful in reducing the amount of time required for definitive diagnosis, these techniques have not proved suitable for monitoring therapeutic effic