Titlebook: Animal Cell Technology: Challenges for the 21st Century; Proceedings of the j Kouji Ikura,Masaya Nagao,Ryuzo Sasaki Conference proceedings

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Metabolic Flux Distributions in Hybridoma Cells at Different Metabolic Statesure by controlling the level of glucose. When cultivated in continuous cultures, these different metabolic states result in multiple steady states marked by different cell, residual nutrient and metabolite concentrations. Most notably, the ratio of lactate produced to glucose consumed was markedly d

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Growth Stimulation of CHO Cells by Phospholipids in Serum-Free Cultureuated by MTT assay. Supplementing PA or LPA to a protein-free basal synthetic medium or a serum-free medium containing insulin and transferrin markedly stimulated the growth of CHO cells, suggesting that PA and LPA are promising growth-promoting supplements for use in constituting a low-protein seru

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Ehancement of Antibody Productivity in the Near-Zero Specific Growth Rate During Glucose and Glutami in the fed-batch culture. From the experiments, it was found that there were two different trends in the relationship between the antibody productivity and the specific growth rate. The specific antibody productivity (g/gCel/hr) was found to significantly increase (327 % over the average of other g

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Long-Term Culture of CHO Cells on Porous Microcarriers in a Stirred Tank Equipped with a Modified Ce20L Biostat UC20 stirred tank reactor The reactor was equipped with bubble-free silicone tubing oxygenator, and a modified perfusion system which can be washed-out backward was substitute for spin-filter to prevent filter clogging During 100 days culture with 0 1% fetal bovine serum (FBS) medium the

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