Titlebook: Animal Cell Technology: Challenges for the 21st Century; Proceedings of the j Kouji Ikura,Masaya Nagao,Ryuzo Sasaki Conference proceedings

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Sebastian Thiede,Christoph Herrmann developed. The model describes the production of cellular macromolecules and monoclonal antibody, the metabolism of glucose and glutamine with the production of lactate and ammonia, and the profiles of cell growth in batch and fed-batch culture. Equations describing the cells arrested in G1 phase (

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Policy strategies to promote eco-innovation,was introduced into Chinese Hamster Ovary (CHO) cells by the electroporation method. The recombinant bovine lactoperoxidase (rbLPO) expressed in a large- scale production system was purified by a combination of anion-exchange chromatography and cation-exchange chromatography. The purified rbLPO was

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Growth Stimulation of CHO Cells by Phospholipids in Serum-Free Cultureuated by MTT assay. Supplementing PA or LPA to a protein-free basal synthetic medium or a serum-free medium containing insulin and transferrin markedly stimulated the growth of CHO cells, suggesting that PA and LPA are promising growth-promoting supplements for use in constituting a low-protein serum-free medium.

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Transient Transfection Induces Early Cytosolic Calcium Signaling in CHO-K1 Cells time “soothed” their Ca. signaling, reducing the oscillations. Time lapse studies revealed shock induced morphological changes in the endoplasmic reticulum of CHO cells. Our finding points to intracellular Ca. signaling as a possible early indicator of the transient transfection fate of CHO-K1 cell

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An On-Line Feeding Strategy for Fed-Batch and Dialysis Cultures of Hybridoma Cellsd in a ten-fold increase of the antibody concentration compared to the batch. Amino acid concentrations were analysed to identify limiting conditions during the cultivation and to analyse the performance of the nutrient supply in the fed-batch and dialysis fed-batch. The potential of the dialysis fe