Titlebook: Animal Cell Biotechnology; Methods and Protocol Ralf Pörtner Book 2014Latest edition Springer Science+Business Media, LLC 2014 DNA profilin

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NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactorsroscopy has played an important role, largely because it requires minimal sample preparation, does not require chromatographic separation, and is quantitative. The concentrations of large numbers of small molecules in the extracellular media or within the cells themselves can be measured directly on

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https://doi.org/10.1007/978-3-319-77069-7nds on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evaluation and optimization of a microcarrier culture for the purpose of production of recombinant proteins are described, for both solid and porous microcarriers.

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Eastern Europe in the Postwar Worldparation, spectra acquisition, and integration. The potential of this technique is exemplified by application to cultures of a Chinese hamster ovary (CHO) cell line. The average error associated to this method is under 3% and the limit of quantification for all metabolites analyzed is below 180 μM.

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Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteinsnds on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evaluation and optimization of a microcarrier culture for the purpose of production of recombinant proteins are described, for both solid and porous microcarriers.

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1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cellsparation, spectra acquisition, and integration. The potential of this technique is exemplified by application to cultures of a Chinese hamster ovary (CHO) cell line. The average error associated to this method is under 3% and the limit of quantification for all metabolites analyzed is below 180 μM.

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https://doi.org/10.1007/978-1-349-22299-5cribed, consisting of the development of a feed medium based on spent media analysis and the establishment of a feeding strategy that consists of adding variable volumes of feed media at specific intervals, after off-line measurement of the concentration of a reference nutrient.

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