Titlebook: Animal Cell Biotechnology; Methods and Protocol Ralf Pörtner Book 2014Latest edition Springer Science+Business Media, LLC 2014 DNA profilin

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Screening and Optimization of Chemically Defined Media and Feeds with Integrated and Statistical App supplements are crucial to protein productivity, medium optimization can be labor intensive and time-consuming. In this chapter, we describe some basic concepts in medium development and introduce a rational and rapid workflow to screen and optimize media and feeds. The major goal of medium screeni

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Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteinsferation, and therefore improving both cell and product yields obtained in these cultures. The establishment of a successful microcarrier culture depends on many factors, such as the type of microcarrier, the cells, and the culture conditions. In this chapter, the basic steps required for the evalua

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Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor Systlopment. Many such devices have been reported in the literature, but only a small number are available commercially. Microbioreactors range in complexity from simple plate-based systems to complex automated parallel bioreactors designed to enable the meaningful scale-down of conventional bioprocesse

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Monitoring Cell Growth, Viability, and Apoptosisility in a bioprocess include microscopic counting, electronic particle counting, image analysis, in situ biomass monitoring, and dieletrophoretic cytometry. These methods work most simply when a fixed volume sample can be taken from a suspension culture. Manual microscopic counting is laborious but

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Quenching Methods for the Analysis of Intracellular Metabolites adherent cells is achieved by application of hot air after removal of the supernatant by suction. For suspension cultures, the addition of excess ice-cold saline results in a rapid inactivation of metabolism and significant dilution of extracellular metabolites. Medium carryover is prevented by rin