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Titlebook: All-Optical Methods to Study Neuronal Function; Eirini Papagiakoumou Book‘‘‘‘‘‘‘‘ 2023 The Editor(s) (if applicable) and The Author(s) 202

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https://doi.org/10.1007/978-3-030-45854-6ed using a miniature electrowetting lens. The 2P-FCM enables three-dimensional two-photon optical recording of structure and activity at multiple focal planes in a freely moving mouse. Detailed methods are provided in this chapter on the 2P-FCM design, operation, and software for data analysis.
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Book‘‘‘‘‘‘‘‘ 2023nal Function .is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors. .This is an open access book..
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Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments,austive review of fluorescent indicators and imaging modalities but rather aims to distill the functional imaging principles driving the choice of both. Scientific priorities determine whether the imaging strategy is based on an “optimal fluorescent indicator” or “optimal imaging modality.” The choi
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