Titlebook: All-Optical Methods to Study Neuronal Function; Eirini Papagiakoumou Book‘‘‘‘‘‘‘‘ 2023 The Editor(s) (if applicable) and The Author(s) 202

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Types of Mycorrhizal Associationes of a temporally focused disc, matched to the dimensions of a neuron’s cell body, anywhere within the operating volume of the microscope. However, since improved placement of light, on its own, is not sufficient to allow precise control of neural firing patterns, we also developed and tested optog

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High-Speed All-Optical Neural Interfaces with 3D Temporally Focused Holography,es of a temporally focused disc, matched to the dimensions of a neuron’s cell body, anywhere within the operating volume of the microscope. However, since improved placement of light, on its own, is not sufficient to allow precise control of neural firing patterns, we also developed and tested optog

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披肩

0893-2336 s, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors. .This is an open access book..978-1-0716-2766-2978-1-0716-2764-8Series ISSN 0893-2336 Series E-ISSN 1940-6045

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https://doi.org/10.1007/978-3-642-40096-4 experiment. Starting with the raw image, we present concepts for artifact avoidance, the extraction of fluorescence intensity traces on single-neuron basis, the identification and binarization of putatively action-potential-related calcium transients, and finally ensemble activity analysis.

配偶

https://doi.org/10.1007/978-3-642-86184-0py equipped with Bessel focus scanning technology and widefield fluorescence microscopy with optical sectioning ability, both of which could be combined with optogenetic stimulation for all optical interrogation of neural circuits. Details on their design and implementation, as well as example applications, are presented.