Titlebook: All-Optical Methods to Study Neuronal Function; Eirini Papagiakoumou Book‘‘‘‘‘‘‘‘ 2023 The Editor(s) (if applicable) and The Author(s) 202

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Drawings, Paintings, and Photographs,ical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.

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0893-2336 ation advice from the experts.This book is open access, whic.This open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this

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https://doi.org/10.1007/978-3-642-97253-9ophysical measurements of synthetically induced percepts. We also highlight new methodologies for validating precise control of optical and behavioral manipulations. Finally, we provide a perspective on upcoming developments that are poised to advance the field.

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Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments,h. Scientific priorities determine whether the imaging strategy is based on an “optimal fluorescent indicator” or “optimal imaging modality.” The choice of the first constrains the choice of the second due to each’s contributions to the fluorescence budget and signal-to-noise ratio of time-varying fluorescence changes.

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Illuminating Neural Computation Using Precision Optogenetics-Controlled Synthetic Perception,ophysical measurements of synthetically induced percepts. We also highlight new methodologies for validating precise control of optical and behavioral manipulations. Finally, we provide a perspective on upcoming developments that are poised to advance the field.

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Optogenetics and Light-Sheet Microscopy,ical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.

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