Titlebook: African Swine Fever Virus; Methods and Protocol Christopher L. Netherton Book 2022 The Editor(s) (if applicable) and The Author(s), under e

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Creating a Dialectical Social Scienceis known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, foll

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Ian I. Mitroff,Richard O. Masonon the use of the soluble cytoplasmic fraction of ASFV-infected monkey stable cells (MS). The soluble antigen proteins of ASFV-infected cells are separated by sucrose precipitation centrifugation, and the supernatant above the sucrose layer is used as an ELISA antigen. The test serum sample reacts w

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https://doi.org/10.1007/978-981-16-3276-1f virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such preparations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells.

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Robert F. Kushner,Jeffrey I. Mechanickes such as real-time PCR and virus isolation provide an understanding as to whether viral genome is present or gives a qualitative assessment of live viral presence in a sample respectively, but neither provide a quantitative measurement of live virus. Here we describe a plaque assay for the titrati

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