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Titlebook: African Swine Fever Virus; Methods and Protocol Christopher L. Netherton Book 2022 The Editor(s) (if applicable) and The Author(s), under e

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发表于 2025-3-21 17:22:55 | 显示全部楼层 |阅读模式
期刊全称African Swine Fever Virus
期刊简称Methods and Protocol
影响因子2023Christopher L. Netherton
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: African Swine Fever Virus; Methods and Protocol Christopher L. Netherton Book 2022 The Editor(s) (if applicable) and The Author(s), under e
影响因子.This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...Authoritative and cutting-edge, .African Swine Fever Virus: Methods and Protocols .aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.  .
Pindex Book 2022
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发表于 2025-3-21 22:22:36 | 显示全部楼层
Necropsy Procedures and Evaluation of Macroscopic Lesions of Pigs Infected with African Swine Fevergy, or virology as core basis of scientific research. Necropsies are an essential tool in veterinary pathology for disease investigation and should be conducted in a routine, systematic, and standard approach. An orderly necropsy procedure will allow the prosector (veterinary clinicians or veterinar
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Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses,further our understanding of the virus–host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFV
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Genotyping of African Swine Fever Virus,is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, foll
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Indirect Immunoperoxidase Test (IPT) for Detection of Antibodies Against African Swine Fever Virus hrough the action of the peroxidase enzyme. In this procedure, African green monkey established cell lines, such as Vero or MS, are infected with African swine fever virus (ASFV)-adapted isolates to these cell cultures. The infected cells are fixed and then used as antigens to determine the presence
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