Titlebook: African Swine Fever Virus; Methods and Protocol Christopher L. Netherton Book 2022 The Editor(s) (if applicable) and The Author(s), under e

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African Swine Fever Virus Hemadsorption Inhibition Assay,wing to determine virus infectious titer in hemadsorption unit (HAU) and differentiate virus strains phenotypically. In the meantime, hemadsorption of particular ASFV strain can by inhibited by homologous anti-ASFV serum containing antibody to the serogroup-specific virus protein (CD2v). Here, we de

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Whole Genome Sequencing of African Swine Fever, infectious disease. It enables high-resolution characterization of viral pathogens in terms of properties that include molecular epidemiology, genotype, serotype, and virulence. However, a beginner’s NGS protocol for characterization of African swine fever virus (ASFV) is lacking. Here, we present

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Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses,further our understanding of the virus–host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFV using fluorescence-activated cell sorting (FACS).

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Quantification of ASFV DNA and RNA in , Soft Ticks,se transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.

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