Titlebook: Wnt Signaling; Methods and Protocol Quinn Barrett,Lawrence Lum Book 2016 Springer Science+Business Media New York 2016 Regenerative medicin

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Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extractsent in the early embryo of ., and . egg extracts have been used to recapitulate complex biological reactions such as microtubule dynamics, DNA replication, chromatin assembly, and phases of the cell cycle. Herein, we describe a biochemical method for analyzing β-catenin degradation using radiolabel

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Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells,, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population inter

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