Titlebook: Wnt Signaling; Methods and Protocol Quinn Barrett,Lawrence Lum Book 2016 Springer Science+Business Media New York 2016 Regenerative medicin

苦笑

Prostaglandins

Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis,e of the Wntless (Wls) gene (..). We previously used this approach to delete the Wntless gene by infecting with a Cre-expressing adenovirus, and to evaluate the effects of Wnt signaling loss on osteogenic potential in osteogenic medium with ascorbic acid. This detailed protocol is adaptable to use with any floxed allele.

浮雕宝石

埋葬

,A Simple Method to Assess Abundance of the β-Catenin Signaling Pool in Cells,m of ICAT (.nhibitor of β-.tenin and .cf) as an affinity matrix. This method is more sensitive and quantitative than immunofluorescence and may be useful in studies that implicate TCF-independent signaling events.

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Wnt-Dependent Control of Cell Polarity in Cultured Cells,ream signaling events that regulate these complex cellular processes are still poorly understood. The methods below describe assays to study Wnt5a-induced cell polarity in cultured cells, which may facilitate our understanding of these complex signaling pathways.

吗啡

ovation

The Generation of Organoids for Studying Wnt Signaling,o study cellular signaling pathways, specifically Wnt signaling. Moreover, we provide a detailed protocol for lentiviral transduction and CRISPR/Cas9-mediated genome modification of organoid cultures.

curettage

Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notor Wnt activities. Here, we provide detailed procedures for preparing enzymatic active Tiki and Notum proteins and the in vitro enzymatic reactions. We also describe a metabolic labeling and click chemistry method for detection of Wnt protein acylation.

开玩笑

可耕种

Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo,s. This biochemical assay will facilitate chemically based studies focused on understanding the role of Tankyrase in cell fate reprogramming and tissue homeostasis and provide insights into the potential role of Wnt/β-catenin signaling in these processes.