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Titlebook: Whole Genome Amplification; Methods and Protocol Thomas Kroneis Book 2015 Springer Science+Business Media New York 2015 Circulating Tumor C

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Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis,logenin generating up to 32 different PCR products. After amplification, the PCR products are separated via capillary electrophoresis and analyzed based on the obtained DNA profiles. Isothermal WGA products of good DNA quality will result in DNA profiles with efficiencies of >90 % of the full DNA profile.
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Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplificatiowing single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.
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Thomas Kroneis,Amin El-Heliebiial role just to have nontrivial Lyapunov inequalities. This fact shows a deep difference with respect to the ordinary case. The linear study is combined with Schauder fixed point theorem to provide new conditions about the existence and uniqueness of solutions for resonant nonlinear problems.
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Principles of Whole-Genome Amplification,ailable technologies for whole-genome amplification (WGA), bridging the last 25 years from the first developments to currently applied methods. We will especially elaborate on research application, as well as inherent advantages and limitations of various WGA technologies.
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Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Ampe of pre-enriched samples by means of CellSearch. The techniques described are micromanipulation, FACS, laser capture microdissection, DEPArray, and microfluidic solutions. All platforms are hampered with a low efficiency and differences in hands-on time and costs are the most important drivers for selection of the optimal platform.
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Bias in Whole Genome Amplification: Causes and Considerations, types including individual cells, fossilized remains and entire ecosystems. Multiple methods of WGA have been developed, each with specific strengths and weaknesses, but with a common defect in that each method distorts the initial template DNA during the course of amplification. The type, extent,
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