Overview: Comprehensive guide to developing, running, and analyzing assays to monitor protein secretion from single proteins to proteome scale.Presents a modular approach to experimental design, covering model .Over the past thirty years, many elegant genetic and biochemical approaches have been combined in order to advance the study of protein secretion and the necessary navigation through cell membranes, yet, despite this progress, less than two hundred membrane protein structures are known, nowhere near the complete inventory that the discovered protein export systems suggest. In .Protein S
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