| 书目名称 | Protein Secretion | | 副标题 | Methods and Protocol | | 编辑 | Anastassios Economou | | 视频video | | | 概述 | Comprehensive guide to developing, running, and analyzing assays to monitor protein secretion from single proteins to proteome scale.Presents a modular approach to experimental design, covering model | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Over the past thirty years, many elegant genetic and biochemical approaches have been combined in order to advance the study of protein secretion and the necessary navigation through cell membranes, yet, despite this progress, less than two hundred membrane protein structures are known, nowhere near the complete inventory that the discovered protein export systems suggest. In .Protein Secretion: Methods and Protocols., leading experts in the field provide robust, well-established protocols to elucidate the multiplicity of tools that have been developed to study protein sorting, membrane targeting, transmembrane crossing, and secretion across multiple membranes. With examples involving both prokaryotic and eukaryotic organisms, the volume covers subjects ranging from bioinformatics and proteomics to fundamental enzymology and genetics to cell biology, structural analyses, and biophysics. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters contain introductions to their respective topics, lists of the key materials and reagents, step-by-step, readily reproducible protocols, and detailed notes on troubleshooting and avoiding known pitfalls...Compr | | 出版日期 | Book 2010 | | 关键词 | Chloroplast; Escherichia coli; Lipid; Polyacrylamid-Gelelektrophorese; Proteomics; biochemistry; bioinform | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-60327-412-8 | | isbn_softcover | 978-1-4939-5707-1 | | isbn_ebook | 978-1-60327-412-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2010 |
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发表于 2025-3-21 17:39:08
