Titlebook: Volume Microscopy; Multiscale Imaging w Irene Wacker,Eric Hummel,Rasmus Schröder Book 2020 Springer Science+Business Media, LLC, part of Sp

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Correlative Super Resolution and Electron Microscopy to Detect Molecules in Their Native Cellular Cion for fixation and to preserve fluorescence. Afterwards, samples are embedded in hydrophilic resin, cut into ultrathin sections, and visualized by, for example, direct stochastic optical reconstruction microscopy (dSTORM) followed by transmission electron microscopy. Fluorescence and electron micr

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A Low-Tech Approach to Serial Section Arrays,nd align the knife to the block face. Regarding substrate handling in the knife boat, we introduce a combination of micromanipulators based on a “supporting hand” concept. These also help with smooth retrieval of the section array from the water onto the substrate without damaging the order of the s

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Large Volumes in Ultrastructural Neuropathology Imaged by Array Tomography of Routine Diagnostic Sasample from a patient with a congenital myopathy (nebulin-related myopathy) characterized by the so-called electron-dense nemaline rods in muscle fibers we describe preparation of section arrays and how they are imaged in a FESEM in an automated way using a typical, commercially available software p

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Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automated Tape-Collecdendritic spine that is stimulated with 2-photon glutamate uncaging on a CA1 pyramidal neuron expressing green fluorescent protein (GFP) in organotypic hippocampal slices. Following fluorescence observation under a 2-photon fluorescence microscope, the tissue is processed for EM using pre-embedding

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Transforming FIB-SEM Systems for Large-Volume Connectomics and Cell Biology,oofreading effort. Moreover, by trading off imaging speed, the system can readily be operated at even higher resolutions achieving voxel sizes of 4 × 4 × 4 nm., thereby generating ground truth of the smallest organelles for machine learning in connectomics and providing important insights into cell

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Forget About Electron Micrographs: A Novel Guide for Using 3D Models for Quantitative Analysis of D we developed tools allowing for performing qualitative, visual assessments, as well as quantification directly in 3D. In this chapter we will revise our working pipeline and show a step-by-step guide to analyze our dataset.

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C. Shan Xu,Song Pang,Kenneth J. Hayworth,Harald F. Hess hängt diese Qualität nicht von der Häufigkeit des Auftretens eines Phänomens ab, sondern vielmehr vom nötigen Spürsinn zum Auffinden des Allgemeinen in jedem einzelnen Fall, von der Phantasie, dem „Freilegen-Können von wirklichen, produktiven Fragen“ (. 1966: 107f).